Natural Environment Monitoring 2011, No 12, 133-136
THE INFLUENCE OF CATACYN AND BENZONAL ON CA2+ ACCUMULATING CAPACITY OF LIVER MITOCHONDRIA IN RATS INTOXICATED WITH THE VENOM OF NAJA OXIANA EICHWALD
The present work describes the influence of benzonal and catacyn (substances which are known by antihypoxic effect) on calcium transport in mitochondria of liver cells of rats intoxicated by Central Asian Cobra venom. White rats (average weight 200–230 g) were used in the experimental studies. These animals were divided into four groups. The rats from the first, second and third groups were injected with the venom of Naja oxiana at 160 g•kg-1 of weight, intramuscularly. Two minutes later the rats from the second and third groups were injected with 50 mg•kg-1 of catacyn or benzonal. The fourth group was injected with the normal saline solution. The rats were decapitated 15 min after venom injection.
It was established that after the injection of benzonal and catacyn into the rats, the Ca2+ accumulating capacity of liver mitochondria decreased. So, after the injection of 50 mg of benzonal per 1 kg of body weight the Ca2+ capacity of rat liver mitochondria dropped by 32.4% of the control level, while catacyn → caused → the drop by 26,8%. The drop of Ca2+ accumulating capacity of mitochondria by above-mentioned antihypoxants is connected with either the → inhibition of Ca2+ ion absorptive function of mitochondria, or with the increase of glycoprotein content by benzonal and catacyn, which specifically binds. Ca2+, or by their activation of ryanodine receptor. The obtained results suggested the inhibition of Ca2+ transport to mitochondria by benzonal and catacyn. It was established that under the venom of Central Asian Cobra effect the consumption of calcium ions in rat liver mitochondria increased by 68,6% from the norm. With benzonal and catacyn it constituted only 17,4% and 20,4%, respectively. It means that the benzonal and catacyn reduce the Ca2+ accumulating capacity of mitochondria, i.e. almost completely reduce the negative effect of N. oxiana venom effect.